Determination of the serum iron-binding capacity
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چکیده
منابع مشابه
Determination of the Serum Iron-binding Capacity.
A colorimetric and an isotopic method for determining the iron-binding capacity are described, using only 1 ml. samples of plasma or serum. Both methods are simple, rapid and sensitive. Satisfactory results are obtained from frozen as well as from fresh samples.
متن کاملSimplified determination of serum iron and total iron-binding capacity.
2,4,6-Tripyridyl-s-triazine (TPTZ) is a color reagent for ferrous ion in the pH range 2 to 10,when ascorbic acid is used as the reducing agent. In this range TPTZ and ferrous ion combine in the ratio of two to one. Tris buffer is commonly used to maintain an alkaline pH, but it reacts with other reducing agents, rendering them ineffective. These observations suggested a method for the joint det...
متن کامل[Determination of serum iron and iron-binding capacity].
Rapid methods depending on differential absorptiometry are described for the determination of the transferrin iron content and the latent iron-binding capacity of blood serum. Each determination requires as little as 0.5 ml. serum. The methods are well adapted for routine use in the ;average' laboratory. Three or four sera may be completely analysed in 30 minutes. All operations are carried out...
متن کاملDetermination of the Iron-binding Capacity of Blood Serum.
A simple experiment was performed to ascertain whether proceduresfor estimating the iron-binding capacity (IBC) of blood serum are quantitative. This was accomplishedby addingf3-globulin (obtained by fractionation of human plasma) to various serum specimens. The 3-globulin solutionswere first assayedfor their IBC. Their addition to serum in these recovery experiments provided data for evaluatio...
متن کاملDetermination of the unsaturated iron-binding capacity of serum.
This paper deals with a modification of the methods suggested by Rath and Finch (1949) and Cartwright and Wintrobe (1949) for the determination of the unsaturated iron-binding capacity of the serum. Transport of iron by the blood plasma may be effected through union with a 8,/-globulin contained in the IV-7 fraction of Cohn (1945); this fraction has been called siderophyllin (Schade and Carolin...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1965
ISSN: 0021-9746
DOI: 10.1136/jcp.18.4.453